Autocytotoxic and autosuppressor T-cell lines generated from autologous lymphocyte cultures

Limiting dilution analyses have demonstrated both the generation and suppression of autocytotoxic cells following in vitro stimulation with autologous peripheral blood mononuclear leukocytes (PBL). Therefore, in order to isolate and characterize the autocytotoxic lymphocytes, interleukin 2-dependent cell lines were derived from autologous mixed lymphocyte microcultures. The cell lines were screened for cytolytic activity against autologous phytohemagglutinin-activated lymphoblasts, autologous and allogeneic B-lymphoblastoid cell lines (B-LCL), and the natural killer target K562. Of 189 cell lines analyzed, 26 demonstrated cytotoxicity against autologous target cells. Cell surface phenotyping of all cell lines indicated that they were of T lymphocyte lineage. Two autocytotoxic T-cell clones were subsequently derived in similar fashion. Cell lines were also screened for autoregulatory activity. Two cells lines were identified that inhibited the generation of autocytotoxicity. Neither of the autoregulatory lines was capable of directly lysing an autocytotoxic line, suggesting that these autosuppressor cells exert their inhibitory effect by a mechanism other than direct lysis of the autocytotoxic effector cell. These findings indicate that through the application of limiting dilution analysis and in vitro cell culture techniques, autocytotoxic and autosuppressor lymphocyte populations can be isolated and utilized to analyze the cellular interactions involved in maintaining self-tolerance.