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Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
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Structural studies of the cell wall polysaccharide from Lactococcus lactis UC509.9
Evgeny Vinogradov1, Irina Sadovskaya2, Thierry Grard2
1National Research Council, 100 Sussex Dr, Ottawa K1A0R6, Canada.
Carbohydrate Research
|March 25, 2018
Summary
Lactococcus lactis cell wall polysaccharides (CWPSs) vary structurally between strains, influencing bacteriophage interactions. This study details the UC509.9 strain
Area of Science:
- Microbiology
- Bacteriology
- Dairy Science
Background:
- Lactococcus lactis is a key starter culture in dairy production.
- Cell wall polysaccharides (CWPSs) in L. lactis act as bacteriophage receptors.
- Structural diversity of CWPSs contributes to the narrow host range of lactococcal bacteriophages.
Purpose of the Study:
- To elucidate the structure of the cell wall polysaccharide (CWPS) from Lactococcus lactis strain UC509.9.
- To understand the role of UC509.9 CWPS in its interaction with the P335-type phage Tuc2009.
Main Methods:
- Methylation analysis
- Deacetylation/deamination
- Smith degradation
- 2D NMR spectroscopy
Main Results:
- The CWPS of L. lactis UC509.9 features a linear tetrasaccharide backbone.
- This backbone is partially substituted with a branched, phosphorylated oligosaccharide.
- The structure includes a common trisaccharide and three non-stoichiometric substitutions.
Conclusions:
- The detailed structure of L. lactis UC509.9 CWPS has been determined.
- This structural information provides insights into phage-host interactions in dairy starter cultures.
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