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Updated: Feb 12, 2026

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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Published on: July 3, 2016

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Site-directed saturation mutagenesis of polyhydroxylalkanoate synthase for efficient microbial production of

Chiaki Hori1, Kenta Oishi2, Ken'ichiro Matsumoto1

  • 1Division of Applied Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, Sapporo 060-8628, Japan; CREST-JST, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.

Journal of Bioscience and Bioengineering
|March 26, 2018
PubMed

Keywords:
BioplasticChiral polymerDirected evolutionLactate-polymerizing enzymePoly[(R)-2-hydroxybutyrate]Polyhydroxybutyrate

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