Related Experiment Video For Catecholamines
Updated: Feb 12, 2026

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
Published on: July 10, 2021
An in vitro study on noradrenergic modulation of final oocyte maturation in the catfish Heteropneustes fossilis
Abha Mishra1, Radha Chaube2, K P Joy3
1Department of Applied Animal Sciences, Babasaheb Bhimrao Ambedkar University, Lucknow, 226025, India.
Abstract:
This study was aimed to elucidate in vitro the effects of endogenous catecholamines: dopamine (DA), noradrenaline (NA) and adrenaline (A), and the β-adrenergic blocker propranolol on induction of final oocyte maturation (FOM) in the catfish Heteropneustes fossilis. With this aim, post vitellogenic follicles from sexually mature gravid female catfish were incubated with each of DA, NA and A in a concentration range of 5-250 μM, and propranolol in a concentration range of 1-200 μg/mL at time points varying from 0 to 30 h). Translucent follicles without germinal vesicle (GV) and opaque follicles with GV were scored separately for the calculation of percentage germinal vesicle breakdown (GVBD), an index of FOM. Data were analyzed by one-way ANOVA and were considered statistically significant when P values were less than 0.05. The analysis of the data showed that the incubation with NA only stimulated GVBD in a concentration - and time-dependent manner. Though the incubation with propranolol decreased total follicular cAMP level significantly at and above 10 μg/mL concentrations, a significant effect of the GVBD increase was noticed at 50 μg/mL or higher. However, the 10 μg/mL concentration of propranolol was effective to inhibit the NA-induced GVBD significantly albeit at a low level (39%). The present study suggests that final oocyte maturation is modulated by NA through a β-adrenergic mechanism, implicating a neural control of oocyte maturation and ovulation in teleosts.
More Related Videos
10:39Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
Published on: April 22, 2017
09:22Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
Published on: February 9, 2015
Related Concept Videos
Maturation of Endosomes
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
Bacterial Protein Maturation
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Case Studies
In-vitro Mutagenesis
In Vitro Fertilization
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...