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Complete genome sequence analysis of PS2, a novel T4-like bacteriophage that infects Serratia marcescens clinical

Tieshan Teng1, Gai Zhang2, Xiangyu Fan3

  • 1Institute of Biomedical Informatics, School of Basic Medical Sciences, Henan University, Kaifeng, 475004, China.

Archives of Virology
|March 26, 2018
PubMed

Insights

Researchers isolated and characterized a lytic phage, vB_SmaM_PS2 (PS2), that infects Serratia marcescens. This T4-like phage possesses a large double-stranded DNA genome and numerous structural genes.

Area of Science:

  • Microbiology and Virology
  • Bacteriophage Research

Background:

  • Serratia marcescens is a clinically relevant bacterium.
  • Bacteriophages are viruses that infect bacteria and are potential therapeutic agents.

Purpose of the Study:

  • To isolate and characterize a novel lytic bacteriophage targeting Serratia marcescens clinical isolates.
  • To determine the genomic and morphological properties of the isolated phage.

Main Methods:

  • Isolation and purification of a lytic phage from bacterial cultures.
  • Morphological characterization using electron microscopy (implied).
  • Genome sequencing and bioinformatic analysis to predict genes and characteristics.

Main Results:

  • A lytic phage, vB_SmaM_PS2 (PS2), was isolated and characterized.
  • Phage PS2 belongs to the Myoviridae family and has a 167,276 bp double-stranded DNA genome with 41.7% GC content.
  • The genome contains 276 protein-coding genes and 4 tRNA genes, with 131 (47%) identified as T4-like genes involved in DNA replication and virion structure.

Conclusions:

  • Phage PS2 is a novel T4-like bacteriophage capable of infecting Serratia marcescens.
  • The genomic features suggest PS2 is a significant addition to the study of T4-like phages infecting Serratia.

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