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Related Experiment Videos

Reference values for peripheral blood lymphocytes from Aotus lemurinus ssp. griseimembra (owl monkey).

C W Caldwell, J E Mrema, H M Taylor

    Journal of Medical Primatology
    |January 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

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    Researchers identified specific monoclonal antibodies (MoAb) that can identify immune cell types in Aotus lemurinus primates. This provides a valuable tool for studying primate immunity and diseases relevant to humans.

    Area of Science:

    • Immunology
    • Primate immunology
    • Cell surface antigen research

    Background:

    • Monoclonal antibodies (MoAb) are crucial for identifying immune cell populations.
    • Aotus lemurinus primates serve as a valuable model for human diseases.
    • Characterizing immune cell subsets in Aotus is essential for translational research.

    Purpose of the Study:

    • To test the reactivity of human-defined monoclonal antibodies against Aotus lemurinus lymphocytes.
    • To identify specific monoclonal antibodies that can reliably mark immune cell subsets in Aotus.
    • To establish reference values for these immune cell subsets in Aotus.

    Main Methods:

    • Peripheral blood lymphocytes from Aotus lemurinus ssp. griseimembra were isolated.
    • Reactivity of various monoclonal antibodies (MoAb) targeting human lymphocyte cell-surface antigens was assessed.

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  • Antibody reactivity patterns were compared to known human immune cell markers.

    • Main Results:

    • Several monoclonal antibodies demonstrated reactivity with Aotus lymphocytes, mirroring human reactivity patterns.
    • Identified MoAb successfully marked pan-T, helper/inducer, suppressor/cytotoxic, pan-B, and natural killer cells in Aotus.
    • Reference values for these identified immune cell subsets in Aotus were determined.

    Conclusions:

    • The characterized monoclonal antibodies are effective tools for identifying key immune cell populations in Aotus lemurinus.
    • These findings facilitate further phenotypic and functional analysis of the immune system in this primate model.
    • This research supports the use of Aotus lemurinus as a model for studying human immune responses and diseases.