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Phosphoproteomic profiling reveals ABA-responsive phosphosignaling pathways in Physcomitrella patens.

Anna Amagai1, Yoshimasa Honda1, Shinnosuke Ishikawa1

  • 1Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo, 184-8588, Japan.

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Summary

Abscisic acid (ABA) signaling is crucial for land plant survival. This study reveals conserved ABA pathways in basal land plants like Physcomitrella patens, alongside unique bryophyte-specific adaptations in ABA phosphosignaling.

Keywords:
Physcomitrella patensabscisic acidphosphoproteomicsprotein kinaseprotein phosphataseprotein phosphorylation

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Area of Science:

  • Plant Biology
  • Molecular Signaling
  • Evolutionary Biology

Background:

  • Abscisic acid (ABA) is vital for terrestrial plant adaptation.
  • Understanding ABA signaling in basal land plants provides evolutionary insights.

Purpose of the Study:

  • To investigate the ABA signaling network in Physcomitrella patens using phosphoproteomics.
  • To identify ABA-responsive phosphopeptides and signaling components in this basal land plant.

Main Methods:

  • Phosphoproteomic analysis of wild-type and ABA-responsive mutant Physcomitrella patens.
  • Quantitative analysis of phosphopeptides to identify ABA-responsive changes.
  • Comparative analysis with known ABA signaling components.

Main Results:

  • Identified 4630 phosphopeptides, with 143 showing ABA responsiveness.
  • Demonstrated conserved SnRK2-mediated phosphorylation and target motifs in bryophytes.
  • Revealed ABA-induced phosphorylation of PpSnRK2B and AREB/ABF transcription factors, regulated by ARK.
  • Showcased the diversity of the ABA-responsive phosphoproteome and unique roles of PP2C and ARK in P. patens.

Conclusions:

  • The core ABA signaling pathway is essential across all land plants.
  • Physcomitrella patens exhibits unique ABA-responsive phosphosignaling pathways, reflecting bryophyte evolution.
  • ABA signaling in basal land plants is a blend of conserved and novel mechanisms.