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Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test
Published on: March 23, 2013
High temporal resolution gastric emptying breath tests in mice
K E Miller1, Ž Bajzer1,2, S S Hein1
1Department of Physiology and Biomedical Engineering and Enteric NeuroScience Program, Mayo Clinic College of Medicine, Rochester, MN, USA.
New high temporal resolution breath tests accurately measure gastric emptying in mice. These assays reveal two-phase gastric emptying is common and influenced by factors like diabetes and hormones.
Area of Science:
- Physiology
- Gastroenterology
- Analytical Chemistry
Background:
- Gastric emptying is a crucial physiological process for digestion, but current measurement methods may not fully capture its complexities.
- Disruptions in gastric emptying are linked to various digestive diseases.
Purpose of the Study:
- To develop and validate high temporal resolution gastric emptying breath tests in mice.
- To investigate the mechanisms and prevalence of altered gastric emptying using these novel assays.
Main Methods:
- Development of solid and liquid gastric emptying breath tests in mice utilizing [13C]-octanoic acid and off-axis integrated cavity output spectroscopy (OA-ICOS).
- Application of stretched gamma variate and 2-component stretched gamma variate models to analyze breath excretion data.
- Utilizing pharmacological (atropine) and physiological (nutrients, cold stress, diabetes, PYY administration) manipulations to assess assay sensitivity.
Main Results:
- The developed assays demonstrated high temporal resolution, accurately detecting both acceleration and delay in gastric emptying.
- Two-component excretion curves, indicative of complex gastric emptying, were more prevalent in diabetic mice and in the C57Bl6 strain compared to Balb/c mice.
- Meal size reduction accelerated gastric emptying in C57Bl6 mice, while PYY administration induced two-phase emptying in Balb/c mice.
Conclusions:
- High temporal resolution gastric emptying assays are effective tools for studying this physiological process.
- Two-phase gastric emptying is more common than previously recognized and may involve intestinal feedback mechanisms.
- While two-phase emptying occurs, its contribution to the overall rate of gastric emptying appears minimal.
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