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Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
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Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

Published on: July 6, 2012

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Ribosome display and selection of single-chain variable fragments effectively inhibit growth and progression of

Shangke Huang1, Lu Feng1, Gaili An2

  • 1Department of Medical Oncology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Cancer Science
|March 26, 2018
PubMed

Insights

Dynamic screening identified single-chain variable fragments (scFvs) with high affinity to cancer stem cells (CSCs). These scFvs, combined with chemotherapy, effectively inhibited tumor progression in vitro and in vivo.

Area of Science:

  • Oncology
  • Immunotherapy
  • Biotechnology

Background:

  • Cancer stem cells (CSCs) are crucial in tumor initiation and metastasis.
  • Identifying CSC surface markers is vital for diagnosis and treatment.
  • Dynamic screening effectively identifies evolving CSC markers.

Purpose of the Study:

  • To dynamically screen single-chain variable fragments (scFvs) targeting cancer stem cells (CSCs).
  • To evaluate the therapeutic potential of selected scFvs in combination with chemotherapy.

Main Methods:

  • Dynamic screening of scFvs against microspheres representing CSCs.
  • In vitro evolution of scFvs to enhance antitumor properties.
  • In vitro and in vivo assessment of scFv efficacy, alone and with chemotherapy.

Main Results:

  • Selected scFvs demonstrated high affinity for microspheres and inhibited their progression.
  • In vitro evolution led to the expression of antitumor-associated proteins.
  • Combined scFv and chemotherapy showed synergistic inhibition of tumor progression.
  • Inhibition involved targeting CD44+/CD24+ and MAPK pathways.

Conclusions:

  • Dynamic screening is a significant approach for identifying effective CSC-targeting agents.
  • Engineered scFvs hold promise for cancer therapy, enhancing chemotherapy efficacy.
  • Targeting CSCs and their collective behaviors offers a novel therapeutic strategy.
Keywords:
CD44+/CD24+microsphereribosome displayscFvscreening

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