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Related Experiment Videos

Two yeast genes that encode unusual protein kinases.

D E Levin, C I Hammond, R O Ralston

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1987
    PubMed
    Summary

    Researchers identified two novel protein kinase genes, KIN1 and KIN2, in yeast. These genes encode unusual mosaic proteins with potential roles in cell signaling, offering new insights into kinase evolution.

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    Area of Science:

    • Molecular Biology
    • Yeast Genetics
    • Protein Kinase Research

    Background:

    • Protein kinases are crucial enzymes regulating cellular processes.
    • Tyrosine-specific protein kinases play key roles in cell signaling pathways.
    • Understanding novel kinase functions is vital for deciphering cellular mechanisms.

    Purpose of the Study:

    • To identify and characterize novel protein kinase genes in Saccharomyces cerevisiae.
    • To investigate the evolutionary relationship of newly discovered kinases to known ones.
    • To analyze the structural features of the identified kinase proteins.

    Main Methods:

    • Degenerate oligonucleotide probes based on conserved tyrosine kinase sequences were synthesized.
    • Probes were used to screen the Saccharomyces cerevisiae genome.

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  • Isolated genes (KIN1 and KIN2) were characterized by DNA sequence analysis.

    • Main Results:

    • Two homologous protein kinase genes, KIN1 and KIN2, were isolated from yeast.
    • KIN1 and KIN2 show homology to bovine cAMP-dependent protein kinase and v-src oncogene.
    • The predicted proteins (117 kDa and 126 kDa) possess N-terminal catalytic domains with mosaic features.
    • These domains exhibit characteristics of both tyrosine-specific and serine/threonine-specific kinases.

    Conclusions:

    • KIN1 and KIN2 represent a novel class of protein kinases in yeast.
    • The mosaic nature of their catalytic domains suggests a unique evolutionary path or functional specialization.
    • These findings expand the known diversity of protein kinases and their potential roles in cellular regulation.