Related Experiment Video
Updated: Feb 12, 2026
12:08
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
5.9K
Complete genome sequence of Bacillus licheniformis BL-010
Sheng He1, Kai Feng1, Tao Ding1
1Institute of Chemistry and Biological Engineering, Department of Biological Technology, University of Science and Technology Beijing, Beijing 100083, People's Republic of China.
Microbial Pathogenesis
|March 27, 2018
Summary
Bacillus licheniformis BL-010 efficiently degrades Aflatoxin B1 (AFB1). Its complete genome sequence reveals genes for enzymes that can detoxify AFB1, supporting biotechnological applications for AFB1 biodegradation.
Area of Science:
- Microbiology
- Genomics
- Biotechnology
Background:
- Aflatoxin B1 (AFB1) contamination poses risks in agriculture and food industries.
- Biodegradation of AFB1 is crucial for mitigating its harmful effects.
- Bacillus licheniformis BL-010 has shown potential for efficient AFB1 degradation.
Purpose of the Study:
- To present the complete genome sequence of Bacillus licheniformis BL-010.
- To identify genes responsible for AFB1 degradation within the BL-010 genome.
- To establish a genomic basis for the biotechnological application of BL-010 in AFB1 detoxification.
Main Methods:
- Isolation of Bacillus licheniformis BL-010 from AFB1-contaminated corn feed.
- Whole-genome sequencing of the BL-010 strain.
- Bioinformatic analysis to identify genes encoding AFB1-degrading enzymes.
Main Results:
- The complete genome of Bacillus licheniformis BL-010 was sequenced, comprising 4,287,714 bp with a 46.12% GC content.
- The genome contains genes encoding enzymes such as laccase and aromatic ring-opening dioxygenase, implicated in AFB1 detoxification.
- The identified genes provide a genetic basis for the strain's AFB1-degrading capabilities.
Conclusions:
- The genome sequence of Bacillus licheniformis BL-010 provides valuable insights into its AFB1 biodegradation mechanisms.
- This genomic information supports the development of Bacillus licheniformis BL-010 as a biocontrol agent for AFB1.
- Further research can leverage these findings for enhanced biotechnological strategies against aflatoxin contamination.
Related Concept Videos
02:02Genomics
40.9K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.9K
02:30Genomic Imprinting and Inheritance
37.3K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.3K
03:21Genome Size and the Evolution of New Genes
9.2K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.2K
02:02Cis-regulatory Sequences
11.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.9K
02:16Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
17.0K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
17.0K
01:29Sequences
295
Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
295