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Updated: Feb 12, 2026

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
Published on: February 16, 2010
Exploring an Artificial Metabolic Route in Fusarium sporotrichioides: Production and Characterization of 7-Hydroxy
Kentaro Kamata1, Hiroki Sato2, Kazuyuki Maeda3,4
1Graduate School of Engineering , Toyo University , 2100 Kujirai , Kawagoe , Saitama 350-8585 , Japan.
Abstract:
An artificial metabolic route to an unnatural trichothecene was designed by taking advantage of the broad substrate specificities of the T-2 toxin biosynthetic enzymes of Fusarium sporotrichioides. By feeding 7-hydroxyisotrichodermin, a shunt pathway metabolite of F. graminearum, to a trichodiene synthase-deficient mutant of F. sporotrichioides, 7-hydroxy T-2 toxin (1) was obtained as the final metabolite. Such an approach may have future applications in the metabolic engineering of a variety of fungal secondary metabolites. The toxicity of 7-hydroxy T-2 toxin was 10 times lower than that of T-2 toxin in HL-60 cells.
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