Related Experiment Video

Updated: Feb 12, 2026

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments
11:24

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

Published on: February 3, 2023

2.5K

Response gene to complement 32 suppresses adipose tissue thermogenic genes through inhibiting β3-adrenergic

Sisi Chen1,2, Xiaohan Mei1, Amelia Yin3,4

  • 1Department of Physiology and Pharmacology, University of Georgia, Athens, Georgia, USA.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|March 27, 2018
PubMed

Insights

Response gene to complement 32 (RGC-32) deficiency enhances thermogenic gene expression in adipose tissues. RGC-32 suppresses this by inhibiting the PI3K/Akt pathway, leading to increased beta3-adrenergic receptor and mTORC1 activity.

Area of Science:

  • Metabolism and Endocrinology
  • Cellular Biology
  • Obesity Research

Background:

  • Response gene to complement 32 (RGC-32) deficiency protects against diet-induced obesity and boosts thermogenic gene expression.
  • The precise mechanisms by which RGC-32 influences thermogenic gene regulation in adipose tissue are not fully understood.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying RGC-32's regulation of thermogenic gene expression in adipose tissues.
  • To investigate the role of RGC-32 in cold-induced adipose tissue browning and its impact on key signaling pathways.

Main Methods:

  • Utilized mouse models with RGC-32 deficiency (Rgc32-/-) and cold exposure.
  • Performed RNA sequencing on inguinal white adipose tissue (iWAT).
  • Analyzed PI3K/Akt signaling, beta3-adrenergic receptor (β3-AR) expression, and mammalian target of rapamycin complex 1 (mTORC1) activity.

Main Results:

  • RGC-32 expression decreased during cold-induced WAT browning.
  • Rgc32-/- mice exhibited increased thermogenic gene expression in iWAT and brown adipose tissue (BAT).
  • RGC-32 deficiency led to decreased Akt phosphorylation, increased β3-AR expression, and enhanced mTORC1 activity, which were crucial for thermogenesis.

Conclusions:

  • RGC-32 suppresses adipose tissue thermogenic gene expression.
  • This suppression occurs via down-regulation of β3-AR expression and mTORC1 activity through a PI3K/Akt-dependent pathway.
  • Targeting RGC-32 may offer a novel strategy for managing obesity and metabolic disorders.
Keywords:
PI3K/AktRGC-32RNA sequencingWAT browningenergy expenditure

More Related Videos

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots08:30

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots

Published on: June 2, 2015

9.8K
Author Spotlight: Optimizing Supraclavicular Brown Adipose Tissue Extraction for Genetic Analysis06:50

Author Spotlight: Optimizing Supraclavicular Brown Adipose Tissue Extraction for Genetic Analysis

Published on: March 29, 2024

3.3K

Related Concept Videos

What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
197.1K
Organization of Genes02:07

Organization of Genes

Overview
73.7K
Gene Flow02:39

Gene Flow

Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
38.1K
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
10.7K
Gene Families01:57

Gene Families

Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
10.0K
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
27.7K