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Related Experiment Video

Updated: Feb 12, 2026

Experimental Generation of Carcinoma-Associated Fibroblasts CAFs from Human Mammary Fibroblasts

Experimental Generation of Carcinoma-Associated Fibroblasts CAFs from Human Mammary Fibroblasts

Published on: October 25, 2011

A Fibroblast Is Not a Fibroblast Is Not a Fibroblast.

Michael S Hu¹, Alessandra L Moore¹, Michael T Longaker²

¹Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, California, USA.

The Journal of Investigative Dermatology

|March 27, 2018

Summary

This summary is machine-generated.

Fibrosis is a major health issue. New research reveals human fibroblast heterogeneity, offering insights into scar formation and potential treatments.

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Last Updated: Feb 12, 2026

Experimental Generation of Carcinoma-Associated Fibroblasts CAFs from Human Mammary Fibroblasts

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Published on: October 25, 2011

Murine Dermal Fibroblast Isolation by FACS

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Published on: January 7, 2016

Establishing Primary Adult Fibroblast Cultures From Rodents

Establishing Primary Adult Fibroblast Cultures From Rodents

Published on: October 5, 2010

Area of Science:

Dermatology
Cell Biology
Medical Research

Background:

Fibrosis following injury presents a significant public health challenge, contributing to disease, death, and substantial healthcare costs.
Recent investigations in mice indicate that dermal fibroblasts exhibit heterogeneity.

Purpose of the Study:

To investigate fibroblast heterogeneity in humans using advanced sequencing techniques.
To enhance the understanding of the cellular mechanisms underlying fibrosis.

Main Methods:

Application of single-cell RNA sequencing (scRNA-seq) technology.
Identification and characterization of distinct fibroblast populations in human skin.

Main Results:

Discovery of major fibroblast subpopulations in human dermis.
Detailed molecular profiling of these identified fibroblast populations.
Establishment of a foundational dataset for human fibroblast heterogeneity.

Conclusions:

Human dermal fibroblasts are more diverse than previously understood.
Understanding fibroblast heterogeneity is crucial for developing targeted therapies for fibrotic diseases.
This research provides a basis for future studies into fibrosis mechanisms and treatments.