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Published on: June 29, 2017
Improved production of isobutanol in pervaporation-coupled bioreactor using sugarcane bagasse hydrolysate in
Hwi-Min Jung1, Ju Yeon Lee1, Jung-Hyun Lee1
1Department of Chemical and Biological Engineering, Korea University, Seongbuk-gu, Seoul 02841, South Korea.
Abstract:
A process of isobutanol production from sugarcane bagasse hydrolysates in Enterobacter aerogenes was developed here with a pervaporation-integrated procedure. Isobutanol pathway was overexpressed in a mutant strain with eliminated byproduct-forming enzymes (LdhA, BudA, and PflB). A glucose-and-xylose-coconsuming ptsG mutant was constructed for effective utilization of lignocellulosic biomass. Toxic effects of isobutanol were alleviated by in situ recovery via a pervaporation procedure. Compared to single-batch fermentation, cell growth and isobutanol titer were improved by 60% and 100%, respectively, in the pervaporation-integrated fermentation process. A lab-made cross-linked polydimethylsiloxane membrane was cast on polyvinylidene fluoride and used in the pervaporation process. The membrane-penetrating condensate contained 55-226 g m-2 h-1 isobutanol with 6-25 g L-1 ethanol after separation. This study offers improved fermentative production of isobutanol from lignocellulosic biomass with a pervaporation procedure.
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