Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Seedless Vascular Plants03:24

Seedless Vascular Plants

67.7K
Seedless Vascular Plants Were the First Tall Plants on Earth
67.7K
Protein Networks02:26

Protein Networks

4.6K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K
Protein Networks02:26

Protein Networks

2.9K
2.9K
Network Covalent Solids02:18

Network Covalent Solids

16.2K
Network covalent solids contain a three-dimensional network of covalently bonded atoms as found in the crystal structures of nonmetals like diamond, graphite, silicon, and some covalent compounds, such as silicon dioxide (sand) and silicon carbide (carborundum, the abrasive on sandpaper). Many minerals have networks of covalent bonds.
To break or to melt a covalent network solid, covalent bonds must be broken. Because covalent bonds are relatively strong, covalent network solids are typically...
16.2K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.4K
Overview
80.4K
Vascular Spasm01:16

Vascular Spasm

3.8K
The vascular phase, also known as vasospasm, is the initial stage of hemostasis, crucial for preventing excessive bleeding when a blood vessel is injured. After a vessel is cut, nerves in the damaged area trigger pain and other sensory impulses. Simultaneously, the smooth muscles in the vessel wall contract, resulting in a vascular spasm. This contraction reduces the vessel's diameter at the injury site, slowing or stopping blood loss through the vessel wall. Vascular spasms typically last...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IntravChip: a vascularized and perfused microfluidic model of the primary tumor microenvironment to collect intravasated tumor cells.

Biofabrication·2026
Same author

4D force patterning enables spatial control of angiogenesis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Self-localized ultrafast pencil beam for volumetric multiphoton imaging.

Nature methods·2026
Same author

Early differential impact of MeCP2 mutations on functional networks in Rett syndrome patient-derived human cortical organoids.

Nature communications·2026
Same author

Quantitative comparison of methods for widespread delivery of small molecules across the blood-brain barrier.

Communications biology·2026
Same author

Innate Immune Evasion of Lyme Disease Pathogen Drives Alzheimer-Like Pathology.

Research square·2026

Related Experiment Video

Updated: Feb 12, 2026

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
08:22

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Published on: August 11, 2017

16.4K

Engineered 3D vascular and neuronal networks in a microfluidic platform.

Tatsuya Osaki1, Vivek Sivathanu1, Roger D Kamm2,3,4

  • 1Department of Mechanical Engineering, Massachusetts institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.

Scientific Reports
|March 28, 2018
PubMed
Summary

Researchers developed a 3D microfluidic model to study neurovascular coupling in motor neuron disease (MND). This model enhances neuronal growth and connectivity, offering new avenues for MND research and drug discovery.

More Related Videos

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
10:53

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Published on: January 3, 2017

10.3K
Perfusable Vascular Network with a Tissue Model in a Microfluidic Device
07:05

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Published on: April 4, 2018

14.9K

Related Experiment Videos

Last Updated: Feb 12, 2026

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
08:22

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Published on: August 11, 2017

16.4K
Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
10:53

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Published on: January 3, 2017

10.3K
Perfusable Vascular Network with a Tissue Model in a Microfluidic Device
07:05

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Published on: April 4, 2018

14.9K

Area of Science:

  • Neuroscience
  • Vascular Biology
  • Biotechnology

Background:

  • Neurovascular coupling is crucial in neurodegenerative diseases like motor neuron disease (MND).
  • In vitro models are vital for understanding MND pathogenesis and for drug screening.
  • Existing models may not fully capture the complex interplay between neurons and vasculature.

Purpose of the Study:

  • To develop and characterize a novel 3D microfluidic model integrating neuronal and microvascular networks.
  • To investigate the bidirectional interactions between neuronal and vascular systems in vitro.
  • To explore the potential of this model for studying neurovascular coupling in MND.

Main Methods:

  • Co-culture of human embryonic stem cell-derived motor neuron (MN) spheroids and endothelial cells (ECs) within microfluidic devices.
  • Establishment of 3D neuronal and microvascular networks under perfusion culture.
  • Assessment of neurite elongation, neuronal connectivity (via Ca2+ oscillations), and vascular network formation.

Main Results:

  • Co-culture with ECs significantly enhanced MN neurite elongation and neuronal connectivity.
  • Improvements in neuronal function were mediated by paracrine factors (e.g., BDNF) and direct cell-cell interactions (delta-notch pathway).
  • Neural networks influenced vascular network formation, indicating bi-directional signaling.

Conclusions:

  • The developed 3D microfluidic model successfully recapitulates neurovascular interactions.
  • This model provides a powerful platform for investigating neurovascular coupling mechanisms relevant to MND pathogenesis.
  • The model holds promise for future drug screening and therapeutic development for neurodegenerative disorders.