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In Vitro Activity of Linezolid, Daptomycin and N-acetylcysteine Agents on Staphylococcus Aureus Biofilms in the

Tuba Kuruoglu1, Mehmet Emin Onger, Derya Bayirli Turan

  • 1Ondokuz Mayis University, School of Medicine, Department of Clinical Microbiology and Infectious Diseases, Samsun, Turkey.

Turkish Neurosurgery
|March 28, 2018
PubMed
Abstract

Insights

N-acetylcysteine (NAC) with daptomycin (DAPT) showed the best bacterial killing effects against methicillin-sensitive Staphylococcus aureus (MSSA) biofilms. However, no significant difference in biofilm formation was observed with different treatments.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Pharmacology

Background:

  • Biofilm formation by Staphylococcus aureus is a significant challenge in medical device infections.
  • Antibiotic resistance and treatment efficacy for methicillin-sensitive Staphylococcus aureus (MSSA) require further investigation.

Purpose of the Study:

  • To evaluate N-acetylcysteine (NAC) alone and with linezolid (LIN) and daptomycin (DAPT) against MSSA biofilms.
  • To assess the impact of these combinations on preventing and eradicating biofilm formation on shunts.

Main Methods:

  • Standard ventriculoperitoneal shunts were infected with MSSA.
  • Microbiological and electron microscopy were used to evaluate biofilm capacity.
  • NAC, LIN, and DAPT were tested individually and in combination.

Main Results:

  • No significant difference in MSSA biofilm formation was observed across different treatment groups.
  • The combination of N-acetylcysteine (NAC) and daptomycin (DAPT) demonstrated the highest bactericidal activity.

Conclusions:

  • Bacterial resistance and antibiotic dose-dependency are critical factors in treatment outcomes.
  • Combination therapy with NAC and DAPT shows promise for enhanced bactericidal effects against MSSA.

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