Related Experiment Video
Updated: Feb 12, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
Published on: November 20, 2013
3D Interconnected Mesoporous Alumina with Loaded Hemoglobin as a Highly Active Electrochemical Biosensor for H2 O2
Xuanyu Yang1, Xiaowei Cheng1, Hongyuan Song2
1Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, iChEM, Fudan University, Shanghai, 200433, China.
Abstract:
Alumina is one of the most common and stable metal oxides in nature, which has been developed as a novel adsorbent in enrichment of biomolecules due to its excellent affinity to phosphor or amino groups. In this study, ordered mesoporous alumina (OMA) with interconnected mesopores and surface acidic property is synthesized through a solvent evaporation induced co-assembly process using poly(ethylene oxide)-b-polystyrene (PEO-b-PS) diblock copolymer as a template and aluminium acetylacetonate (Al(acac)3 ) as the aluminium source. The pore size (12.1-19.7 nm), pore window size (3.5-9.0 nm) and surface acidity (0.092-0.165 mmol g-1 ) can be precisely adjusted. The highly porous structure endows the OMA materials with high hemoglobin (Hb) immobilization capacity (170 mg g-1 ). The obtained Hb@OMA composite is used as an electrocatalyst of biosensor for convienet and fast detection of hydrogen peroxide (H2 O2 ) with a low H2 O2 detection limit of 1.7 × 10-8 m and a wide linear range of 2.5 × 10-8 to 5.0 × 10-5 m. Moreover, the Hb@OMA sensors show a good performance in real time detection of H2 O2 released from Homo sapiens bone osteosarcoma, indicating their potential application in complex biological processes.
Related Concept Videos
What is an Electrochemical Gradient?
The chemical gradient relies on differences in the abundance of a substance on the outside versus the inside of a cell and flows from areas of high to low ion concentration. In contrast, the electrical gradient revolves around an...
Hemoglobin
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
Secondary Active Transport
Interfacial Electrochemical Methods: Overview
Impact Loading
In cases of elastic deformation,...
Primary Active Transport

