Location of genes D16, D17, and N4 encoding tail proteins on the physical map of bacteriophage T5

Bacteriophage T5 DNA fragments were cloned into plasmid pBR322. Recombinant plasmids complementing T5 amber mutants were isolated, and used as hybridization probes with T5 DNA in Southern blots. Employing this approach the three T5 genes D16, D17, and N4 were mapped with respect to the physical map of T5, and shown to be located at 74%, 72%, and 82% of the genome, respectively.