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Related Experiment Video

Updated: Feb 12, 2026

Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model

Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model

Published on: June 11, 2017

An Engineered Complement Factor H Construct for Treatment of C3 Glomerulopathy.

Yi Yang¹, Harriet Denton¹, Owen R Davies²

¹Institute of Cellular Medicine, Newcastle University and National Renal Complement Therapeutics Centre, Royal Victoria Infirmary, Newcastle upon Tyne, UK.

Journal of the American Society of Nephrology : JASN

|March 29, 2018

Summary

New mini-Factor H constructs show promise for treating C3 glomerulopathy. These engineered proteins demonstrate improved efficacy and longer serum half-life, offering potential new therapeutic options for complement-mediated diseases.

Keywords:

Immunology and pathology complement glomerulopathy membranoproliferative glomerulonephritis (MPGN)

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Last Updated: Feb 12, 2026

Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model

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Published on: June 11, 2017

Fabrication of Myogenic Engineered Tissue Constructs

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Published on: May 1, 2009

Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth

Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth

Published on: February 9, 2012

Area of Science:

Immunology
Nephrology
Biochemistry

Background:

C3 glomerulopathy (C3G) involves complement alternative pathway dysregulation, with limited treatment options.
Full-length Factor H (FH) is a potent regulator but difficult to produce.
Previous mini-FH constructs were effective but had short serum half-lives.

Purpose of the Study:

To develop improved mini-Factor H (FH) constructs with enhanced stability and efficacy.
To investigate the therapeutic potential of dimeric mini-FH variants for C3G.

Main Methods:

Engineered two homodimeric mini-FH constructs (FH ^{R1-2^1-5^18-20} and FH ^{1-5^18-20^R1-2}) by adding an oligomerization domain to a previously developed mini-FH.
Assessed construct binding, complement inhibition in vitro, and efficacy in FH-deficient mice.

Main Results:

Both dimeric constructs showed avid binding to C3b and superior inhibition of complement activity compared to native FH.
FH ^{1-5^18-20^R1-2} exhibited a >5-fold longer serum half-life and better kidney retention than the non-dimeric version.
FH ^{1-5^18-20^R1-2} significantly reduced glomerular C3 deposition in vivo.

Conclusions:

The dimeric mini-FH construct FH ^{1-5^18-20^R1-2} demonstrates enhanced stability and efficacy.
This construct holds potential as a therapeutic agent for C3G and other complement-mediated kidney diseases.