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Updated: Feb 12, 2026

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
Published on: June 27, 2017
Identification of Response Elements on Promoters Using Site-Directed Mutagenesis and Chromatin Immunoprecipitation
Salah Boudjadi1, Julie C Carrier2, Jean-François Beaulieu3
1Cancer Molecular Pathology Section, Laboratory of Pathology, National Cancer Institute, Bethesda, MD, USA. Salah.Boudjadi@nih.gov.
Abstract:
Proximal promoters are located upstream of the transcription start sites of genes, and they contain regulatory sequences on which bind different transcription factors for promoting colorectal cancer progression. Here we describe the comprehensive methodology used previously for the identification and functional characterization of MYC-responsive elements in the integrin α1 subunit (ITGA1) gene using a combination of in silico analysis, site-directed mutagenesis, and chromatin immunoprecipitation.
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