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Updated: Feb 12, 2026

Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer
Published on: February 10, 2023
Proteomics Analysis of Colorectal Cancer Cells
Anaïs Chauvin1, François-Michel Boisvert2
1Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada.
Abstract:
Proteomics allows the simultaneous detection and identification of thousands of proteins within a sample. Here, we describe a quantitative method to compare protein expression and subcellular localization of different cell lines representative of different stages of colorectal cancer using stable isotope labeling with amino acids in culture, or SILAC. We also describe a biochemical fractionation approach to separate different cellular compartments and the necessary steps to obtain a specific proteomic profile of each cell line. This technique enables a comprehensive proteomic analysis of cancer cell lines and the identification of pathways that are deregulated in different cancer cell lines.
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