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Updated: Feb 12, 2026

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
Published on: January 31, 2025
Proteomic Profiling for Colorectal Cancer Biomarker Discovery
Paula Álvarez-Chaver1, Loretta De Chiara2, Vicenta Soledad Martínez-Zorzano2
1Proteomics Unit, Structural Determination, Proteomics and Genomics Service, CACTI, University of Vigo, Vigo, Spain. paulachaver@uvigo.es.
Abstract:
Nowadays, the ideal biomarker for colorectal cancer (CRC) has not been found. Two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) are suitable techniques for searching new biomarkers. In this chapter, we describe methodology for biomarker discovery based on a proteomic approach. In addition, special attention is given to the sample preparation, including protein extraction, fractionation, and cleanup, as we consider this a critical step. Comparing the proteomic profile of tumor and mucosa, we identified the nucleoside diphosphate kinase A (NDKA) protein as a candidate biomarker for CRC. Finally, we validated NDKA with an ELISA kit using serum samples from individuals of a screening cohort. Our results suggest that serum NDKA is a potential biomarker for screening of CRC and premalignant advanced adenomas (AA).
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