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Updated: Feb 12, 2026

Culture of Bladder Cancer Organoids as Precision Medicine Tools
Published on: December 28, 2021
Use of Organoids to Characterize Signaling Pathways in Cancer Initiation
Christina Oatway1, Calley L Hirsch1, Alex Gregorieff2,3,4
1Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Canada.
Abstract:
The development of intestinal organoid technology has greatly accelerated research in the field of colorectal cancer. Contrary to traditional cancer cell lines, organoids are composed of multiple cell types arranged in 3D structures highly reminiscent of their native tissues. Thus, organoids provide a near-physiological and readily accessible model to study tissue morphogenesis, adult stem cell behavior and tumorigenesis. Here, we provide protocols for establishing intestinal organoid cultures from genetically modified mouse lines and describe methods to overexpress and knockout genes of interest using lentiviral-based approaches.
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