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Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device
Kai-Hong Guo1, Pei-Hsuan Chen1, Chieh Lin1
1Department of Chemistry , National Taiwan Normal University , Taipei 116 , Taiwan.
Abstract:
This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.
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