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A myeloperoxidase precursor, pro-myeloperoxidase, is present in human plasma and elevated in cardiovascular disease

Irada S Khalilova1, Nina Dickerhof1, Tessa J Mocatta1

  • 1Centre for Free Radical Research, Department of Pathology, University of Otago Christchurch, Christchurch, New Zealand.

Plos One
|March 29, 2018
PubMed

Insights

Circulating pro-myeloperoxidase (pro-MPO) was detected in human blood plasma. This precursor enzyme was more prevalent in patients with myocardial infarction, suggesting its role in cardiovascular disease.

Area of Science:

  • Biochemistry
  • Cardiovascular Research
  • Immunology

Background:

  • Myeloperoxidase (MPO)-derived oxidants contribute to tissue damage in inflammatory diseases like cardiovascular disease.
  • Pro-myeloperoxidase (pro-MPO), the precursor to MPO, is secreted by certain cells but its presence in circulation is unconfirmed.

Purpose of the Study:

  • To investigate the presence of pro-myeloperoxidase (pro-MPO) in human blood plasma.
  • To determine if circulating pro-MPO levels differ between patients with myocardial infarction and healthy controls.

Main Methods:

  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.
  • Immunoblot analyses of human blood plasma samples.

Main Results:

  • Pro-myeloperoxidase (pro-MPO) was detected in human blood plasma.
  • Pro-MPO was identified more frequently in plasma from myocardial infarction patients than in control donors.

Conclusions:

  • Circulating pro-MPO is present in human plasma.
  • Elevated pro-MPO in myocardial infarction patients suggests a potential role in cardiovascular disease pathogenesis through protein oxidative modification.

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