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Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

Analyzing Immunoglobulin Repertoires.

Neha Chaudhary¹, Duane R Wesemann¹

¹Division of Rheumatology, Department of Medicine, Immunology and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, United States.

Frontiers in Immunology

|March 30, 2018

Summary

This summary is machine-generated.

High-throughput sequencing of B cell receptor (BCR) genes reveals adaptive immunity. This review covers BCR sequencing methods, data analysis, and interpretation challenges for accurate immune response studies.

Keywords:

B cell repertoire immunoglobulin next-generation sequencing repertoire statistical analysis

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Area of Science:

Immunology
Genomics
Bioinformatics

Background:

Adaptive immune responses rely on diverse T cell receptor (TCR) and B cell receptor (BCR) repertoires.
High-throughput sequencing (HTS) offers powerful tools for studying these repertoires.
Current analysis techniques require refinement to accurately reflect biological phenomena.

Purpose of the Study:

To review current methods and challenges in library preparation, sequencing, and statistical analysis of lymphocyte receptor repertoire studies.
To provide a comprehensive overview of immune repertoire generation, from sample preparation to data interpretation.
To emphasize the specific complexities of immunoglobulin (Ig)/BCR sequence analysis.

Main Methods:

Review of existing literature on lymphocyte receptor repertoire sequencing and analysis.
Discussion of sample preparation techniques.
Exploration of various sequencing platforms and data processing strategies.
Examination of statistical tools for repertoire data analysis.

Main Results:

Identification of key steps and challenges in immune repertoire analysis.
Highlighting the evolution of sequencing and analysis techniques.
Addressing the unique complexities of BCR analysis, including somatic hypermutation and class switch recombination.

Conclusions:

Accurate analysis of lymphocyte receptor repertoires is crucial for understanding adaptive immunity.
Standardization and advancement of library preparation, sequencing, and statistical methods are needed.
Further research focusing on BCR-specific analysis will enhance our comprehension of antibody-mediated immunity.