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An ELISA Assay for Quantifying Monomeric C-Reactive Protein in Plasma.

Lin Zhang1, Hai-Yun Li1, Wei Li1

  • 1MOE Key Laboratory of Environment and Genes Related to Diseases, School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an, China.

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|March 30, 2018
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Summary

Researchers developed a new assay to measure monomeric C-reactive protein (mCRP), a more specific inflammation marker than native C-reactive protein (nCRP). This tool aids in understanding mCRP

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C-reactive proteinautoimmune diseasesinflammationmonomeric C-reactive proteinpsoriasisurticaria

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Area of Science:

  • Biochemistry
  • Immunology
  • Clinical Diagnostics

Background:

  • Native C-reactive protein (nCRP) is a non-specific inflammation marker, often acting as a bystander in chronic diseases.
  • Evidence suggests monomeric C-reactive protein (mCRP) is the active form at inflammatory sites, potentially offering higher specificity.
  • Quantifying mCRP in clinical samples has been challenging due to a lack of suitable assays.

Purpose of the Study:

  • To develop a reliable sandwich ELISA assay for quantifying plasma mCRP.
  • To assess if mCRP is a more specific and causally relevant disease marker than nCRP.
  • To provide a tool for evaluating the clinical utility of mCRP.

Main Methods:

  • Development of a sandwich ELISA assay using commercially available reagents.
  • Validation of the assay for reproducibility and conformation specificity.
  • Determination of the assay's detection limit.

Main Results:

  • A reproducible and highly conformation-specific sandwich ELISA for plasma mCRP was successfully developed.
  • The assay demonstrates a reliable detection limit of 1 ng/mL.
  • mCRP was identified as a potentially superior marker compared to nCRP in certain autoimmune skin disorders.

Conclusions:

  • The developed ELISA assay enables accurate quantification of plasma mCRP.
  • mCRP shows promise as a more specific biomarker for inflammation and pathogenesis than nCRP.
  • This assay is a valuable tool for future clinical studies on mCRP's significance.