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Related Experiment Video

Updated: Feb 12, 2026

An Aptamer-based Sensor for Unchelated GadoliniumIII
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Aptamer based vanillin sensor using an ion-sensitive field-effect transistor.

Alexander Kuznetsov1, Natalia Komarova2, Maria Andrianova2

  • 1Scientific-Manufacturing Complex Technological Center, 1-7 Shokin Square, Zelenograd, Moscow, 124498, Russia. kae@tcen.ru.

Mikrochimica Acta
|March 30, 2018
PubMed
Summary
This summary is machine-generated.

A novel aptasensor was developed for vanillin detection using an ion-sensitive field-effect transistor (ISFET) and a specific DNA aptamer. This biosensor demonstrates high sensitivity and selectivity for vanillin in real samples.

Keywords:
Aptamer, ion-selective field-effect transistorAptasensor, small target, low-weight targetCapture-SELEXVanillin

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Area of Science:

  • Biotechnology and Biosensor Development
  • Analytical Chemistry and Molecular Recognition
  • Materials Science for Sensor Applications

Background:

  • Accurate detection of vanillin is crucial for food quality control and flavor analysis.
  • Existing detection methods often lack sensitivity, selectivity, or require complex sample preparation.
  • Aptasensors offer a promising alternative due to their high specificity and potential for miniaturization.

Purpose of the Study:

  • To develop and characterize a novel aptasensor for the selective detection of vanillin.
  • To utilize an ion-sensitive field-effect transistor (ISFET) platform for sensitive signal transduction.
  • To validate the aptasensor's performance in complex matrices such as coffee extract.

Main Methods:

  • Selection of a high-affinity DNA aptamer for vanillin using the Capture-SELEX protocol.
  • Immobilization of the selected aptamer (Van_74) onto the Ta2O5-sensitive surface of an ISFET via click chemistry.
  • Development of a detection scheme based on vanillin-induced dehybridization of an ssDNA probe, leading to a change in surface potential detected by the ISFET.

Main Results:

  • The aptamer Van_74 exhibited specific binding to vanillin with a dissociation constant of >7.8 μM and demonstrated selectivity against potential interferents.
  • The developed ISFET-based aptasensor achieved a low detection limit of 1.55 × 10^-7 M and a dynamic range from 1.55 × 10^-7 M to 1 × 10^-6 M.
  • The aptasensor successfully detected vanillin in coffee extract samples, demonstrating its applicability in real-world scenarios.

Conclusions:

  • The developed ISFET aptasensor provides a sensitive and selective platform for vanillin detection.
  • The Capture-SELEX protocol is effective for generating aptamers against small molecules like vanillin.
  • This aptasensor technology holds potential for rapid and reliable analysis of vanillin in various applications.