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Decreased hematopoietic accessory cell function following bone marrow transplantation.
S G Emerson1, C A Sieff, R G Gross
1Division of Pediatric Hematology/Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts.
Experimental Hematology
|November 1, 1987
Summary
Bone marrow transplantation impairs accessory cell function, reducing hematopoietic growth factors. This defect, particularly in T cells, may stem from generalized lymphocyte dysfunction post-transplant.
Area of Science:
- Hematology
- Immunology
- Stem Cell Biology
Background:
- Hematologic engraftment after bone marrow transplantation (BMT) depends on stem cells and accessory cells.
- Accessory cells produce trophic factors crucial for stem cell proliferation and differentiation into mature blood cells.
Purpose of the Study:
- To investigate hemopoietic accessory cell function in BMT recipients.
- To understand the regulation of hematopoiesis following transplantation.
Main Methods:
- Studied accessory cell function in BMT recipients from 3 weeks to 10 months post-transplant.
- Fractionated accessory cells to assess burst-promoting activity (BPA) and granulocyte-macrophage colony-stimulating activity (GM-CSA).
- Utilized limiting-dilution analysis (LDA) for T-cell precursors.
Main Results:
- Accessory cell function was generally decreased post-BMT.
- Fc receptor-positive (Fc+) cells and T cells showed significantly reduced BPA and GM-CSA production.
- Deficiencies were corrected by soluble factors, indicating a failure in trophic hormone production.
- Reduced frequency of phytohemagglutinin-responsive T-cell precursors was observed.
Conclusions:
- Bone marrow transplantation leads to impaired accessory cell function, particularly in T cells.
- Deficient production of hematopoietic growth factors by accessory cells contributes to post-transplant hematopoiesis issues.
- This defect may reflect broader lymphocyte dysfunction in BMT patients.