Nanozymatic Antioxidant System Based on MoS₂ Nanosheets.

Area of Science:

• Materials Science
• Biochemistry
• Nanotechnology

Background:

• Aerobic cells rely on the enzymatic antioxidant system (EAS) for protection against oxidative stress.
• The EAS is vulnerable to excessive reactive oxygen species (ROS) production.
• Developing robust antioxidant systems is crucial for combating oxidative damage.

Purpose of the Study:

• To investigate the antioxidant properties of molybdenum disulfide (MoS2) nanosheets.
• To establish a nanozyme antioxidant system (NAS) using MoS2 nanosheets.
• To evaluate the efficacy of MoS2-based NAS in protecting against oxidative stress in vitro and in vivo.

Main Methods:

• MoS2 nanosheets were synthesized and characterized.
• The enzyme-mimicking activities (SOD, CAT, POD) of MoS2 nanosheets were assessed under physiological conditions.
• The radical scavenging capabilities of MoS2 nanosheets against hydroxyl, DPPH, and nitric oxide radicals were evaluated.
• In vivo protection against hydrogen peroxide (H2O2)-induced oxidative injury was tested using Escherichia coli, Staphylococcus aureus, and A549 cell models.

Main Results:

• MoS2 nanosheets exhibited intrinsic superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD)-like activities.
• MoS2 nanosheets demonstrated POD-like activity via electron transfer without generating ROS.
• A nanozyme antioxidant system (NAS) was established, effectively scavenging various free radicals.
• MoS2 nanosheets provided superior protection to bacteria and cells against H2O2-induced oxidative damage.

Conclusions:

• MoS2 nanosheets function as a versatile nanozyme antioxidant system (NAS).
• The NAS effectively regulates oxidative stress and protects against oxidative injury.
• MoS2 nanosheets hold significant potential as antioxidants in pathological conditions and for applications in biocatalysis and nano-biomedicine.