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Genotypic characterization of Haemophilus influenzae isolates from paediatric patients in Japan

Yoko Adachi1, Mariko Ando1, Miyuki Morozumi1

  • 1Department of Infectious Diseases, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.

Journal of Medical Microbiology
|March 30, 2018
PubMed

Insights

β-lactamase-negative ampicillin-resistant (BLNAR) Haemophilus influenzae is common in respiratory infections. This study genetically characterized BLNAR isolates, revealing diverse strains in pneumonia and acute otitis media, unlike the predominant Hib in meningitis.

Area of Science:

  • Medical Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • β-lactamase-negative ampicillin-resistant (BLNAR) *Haemophilus influenzae* is a significant pathogen, particularly in Japan.
  • BLNAR strains pose challenges in treating respiratory tract infections.

Purpose of the Study:

  • To genetically characterize BLNAR *Haemophilus influenzae* isolates.
  • To determine associations between genotypic characteristics and resistance patterns.
  • To investigate the epidemiology of BLNAR *H. influenzae* in paediatric infections.

Main Methods:

  • Collected 191 *H. influenzae* isolates from paediatric pneumonia, acute otitis media (AOM), and meningitis cases (2000-2011).
  • Performed BLNAR-PCR for β-lactam resistance and multilocus sequence typing (MLST) for genotypic characterization.
  • Analyzed sequence types (STs) using eBURST v3.

Main Results:

  • 46.1% (88/191) of isolates were BLNAR.
  • BLNAR strains were prevalent in pneumonia (60.7%) and AOM (48.5%) but less so in meningitis (29.0%).
  • MLST revealed genetic diversity in non-typeable *H. influenzae* (NTHi) from pneumonia and AOM, with specific STs associated with BLNAR. Meningitis cases were dominated by ST54, distinct from the common ST6 in databases.

Conclusions:

  • Non-typeable *H. influenzae* (NTHi) from pneumonia and AOM exhibit significant genetic diversity.
  • *Haemophilus influenzae* type b (Hib) remains predominant in meningitis cases.
  • Confirmed associations between specific STs and β-lactam resistance in NTHi.
Keywords:
BLNARH. influenzae type b, HibHaemophilus influenzaemultilocus sequence typing, MLSTnon-typeable H. influenzae, NTHi

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