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Updated: Feb 12, 2026
08:23
Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
Published on: March 2, 2020
13.6K
Unique Fluorescent Imaging Probe for Bacterial Surface Localization and Resistant Enzyme Imaging.
Hui Ling Chan1, Linna Lyu1, Junxin Aw1
1Division of Chemistry and Biological Chemistry, School of Physical & Mathematical Sciences , Nanyang Technological University , Singapore , 637371 , Singapore.
ACS Chemical Biology
|March 30, 2018
Summary
A novel probe (DFD-1) selectively identifies and localizes drug-resistant bacteria expressing AmpC β-lactamase on their surface. This advancement enables rapid screening for resistant bacterial infections, improving clinical diagnosis and treatment strategies.
Area of Science:
- Biochemistry
- Microbiology
- Medical Imaging
Background:
- Antibiotic resistance poses a significant global health challenge, complicating bacterial infection treatment.
- Accurate and timely diagnosis of resistant bacteria is crucial for effective clinical management.
Purpose of the Study:
- To develop a specific method for selective visualization of drug-resistant bacteria.
- To create a probe capable of recognizing AmpC β-lactamase and localizing to the bacterial surface.
Main Methods:
- Development of an enzyme-responsive cephalosporin probe (DFD-1) utilizing fluorescence resonance energy transfer (FRET) with an "off-on" mechanism.
- Utilized a lipid-azide conjugate (LA-12) for bacterial surface localization via copper-free click chemistry.
- Demonstrated selective recognition of AmpC β-lactamase over other enzymes like TEM-1 β-lactamase.
Main Results:
- DFD-1 probe showed high selectivity for drug-resistant bacteria harboring AmpC β-lactamase.
- Significant fluorescence enhancement (approximately 67-fold) was observed in the presence of AmpC enzymes.
- Live cell imaging and flow cytometry confirmed the probe's ability to localize and detect resistant bacteria.
Conclusions:
- The developed DFD-1 probe offers a promising tool for selective localization and screening of AmpC resistance bacteria.
- This technology holds potential for advancing clinical microbiological applications and combating antibiotic resistance.
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