Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cellular Differentiation00:57

Cellular Differentiation

5.5K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
5.5K
Cellular Respiration01:18

Cellular Respiration

2.1K
Cellular respiration is a crucial metabolic process through which cells obtain energy from organic substances, mainly glucose, to produce adenosine triphosphate (ATP). This process includes the oxidation of substrates and the transfer of electrons to a separate electron acceptor, facilitating ATP synthesis through a sequence of biochemical reactions.Glycolysis: The Initial StepGlycolysis is the first stage of cellular respiration, occurring in the cytoplasm of both prokaryotic and eukaryotic...
2.1K
In-situ Hybridization02:31

In-situ Hybridization

10.6K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
10.6K
Introduction to Cellular Respiration01:22

Introduction to Cellular Respiration

190.2K
Organisms harvest energy from food, but this energy cannot be directly used by cells. Cells convert the energy stored in nutrients into a more usable form: adenosine triphosphate (ATP).
ATP stores energy in chemical bonds that can be quickly released when needed. Cells produce energy in the form of ATP through the process of cellular respiration. Although much of the energy from cellular respiration is released as heat, some of it is used to make ATP.
During cellular respiration, several...
190.2K
Cellular Membranes and Drug Transport01:24

Cellular Membranes and Drug Transport

1.6K
Drugs must traverse multiple biological barriers, such as multi-layered skin, single-layered intestinal epithelium, and the plasma membrane, to reach their target sites within the body. The plasma membrane, a highly structured composite of phospholipids, carbohydrates, and proteins, is the cell's protective boundary, facilitating selective substance exchange.
Phospholipids arrange themselves into a bilayer, with hydrophilic heads oriented outward and hydrophobic tails facing inward.
1.6K
Inflammatory Response I: Vascular and Cellular01:30

Inflammatory Response I: Vascular and Cellular

16.8K
The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...
16.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Situ Translation of Terminal Glycans into Covalent Readouts in Complex Biological Systems.

Analytical chemistry·2026
Same author

Electron-Ion Coupling for Nanostructured Photocathode in Ion-Assisted Photoelectrochemical Microfluidic Biosensing Platform.

Analytical chemistry·2026
Same author

Identification and quantification of intracellular N-acetylglucosamine modified glycoRNAs.

Nature communications·2026
Same author

Synthetic Antibody Mimetics with ROS-Gated Saccharide Release for Targeted Colitis Therapy.

Advanced healthcare materials·2026
Same author

NIR-II Fluorescent Timer-Embedded Drug for Real-Time Tracing of Immunogenic Cell Death and Guiding Chemo-immunotherapy.

Analytical chemistry·2026
Same author

Metal Defect-Mediated Adsorption-Catalytic Platform Drives Efficient Electrochemiluminescence Sensing.

Analytical chemistry·2026
Same journal

Design Principles for Negative Thermal Expansion in Two-Dimensional Materials.

Accounts of chemical research·2026
Same journal

Main Group Redox Catalysis: New Frontiers with Germanium and Tin.

Accounts of chemical research·2026
Same journal

Taming Irreversibility in sp<sup>2</sup>-Carbon-Conjugated COFs from Polycrystalline Powders to Single Crystals and Thin Films.

Accounts of chemical research·2026
Same journal

Electroactive Imidazolium Ionic Liquids in Organic Synthesis.

Accounts of chemical research·2026
Same journal

Calix[4]resorcinarene-Based Porous Organic Cages: Synthesis and Applications.

Accounts of chemical research·2026
Same journal

Light-Driven Dual Rotary Molecular Motors and Beyond.

Accounts of chemical research·2026
See all related articles

Related Experiment Video

Updated: Feb 12, 2026

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
08:26

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

Published on: June 25, 2018

7.1K

In Situ Cellular Glycan Analysis.

Yunlong Chen1, Lin Ding1, Huangxian Ju1

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering , Nanjing University , Nanjing 210023 , P. R. China.

Accounts of Chemical Research
|March 30, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed novel in situ methods for analyzing cellular glycans, offering real-time, spatial insights crucial for understanding biological processes and advancing tumor diagnosis and treatment.

More Related Videos

Mitochondrial Preparation from Microglia for Glycan Analysis
06:40

Mitochondrial Preparation from Microglia for Glycan Analysis

Published on: May 30, 2025

844
Glycan Node Analysis: A Bottom-up Approach to Glycomics
11:36

Glycan Node Analysis: A Bottom-up Approach to Glycomics

Published on: May 22, 2016

11.2K

Related Experiment Videos

Last Updated: Feb 12, 2026

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
08:26

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

Published on: June 25, 2018

7.1K
Mitochondrial Preparation from Microglia for Glycan Analysis
06:40

Mitochondrial Preparation from Microglia for Glycan Analysis

Published on: May 30, 2025

844
Glycan Node Analysis: A Bottom-up Approach to Glycomics
11:36

Glycan Node Analysis: A Bottom-up Approach to Glycomics

Published on: May 22, 2016

11.2K

Area of Science:

  • Biochemistry and Molecular Biology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Glycans on cell surfaces are vital for biological processes like cell communication and immune response.
  • Aberrant glycan expression in tumor cells offers potential for cancer diagnosis and treatment.
  • Current in vitro methods lack real-time, spatial glycan information from intact cells.

Purpose of the Study:

  • To develop and review in situ analytical methods for cellular glycans.
  • To overcome limitations of traditional lysis-based glycan analysis.
  • To enable real-time, quantitative, and spatial glycan profiling on intact cells.

Main Methods:

  • Electrochemical glycan-recognizable probes for quantification.
  • Multichannel electrodes and lectin probes for simultaneous monitoring.
  • Functional nanoprobes for fluorescence and Raman imaging of glycans and enzymes.
  • Enzyme-based methods for glycan remodeling and imaging.

Main Results:

  • Demonstrated reliability and practicality of developed in situ methods.
  • Enabled dynamic monitoring and visualization of cell surface and intracellular glycans.
  • Provided insights into glycan-related biological processes through drug/gene interference studies.

Conclusions:

  • Developed methods offer a versatile toolkit for in situ glycan analysis.
  • Addresses the need for real-time, spatial glycan information in intact cells.
  • Paves the way for unraveling complex glycan functions and improving tumor diagnostics.