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Updated: Feb 12, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
Published on: November 4, 2016
Pathological Features of Staphylococcus aureus Induced Mastitis in Dairy Cows and
Zhi Chen1,2, Hailei Xia1,2, Hongliang Shen3
1College of Animal Science and Technology , Yangzhou University , Yangzhou 225009 , PR China.
Abstract:
In part as a result of the production of an enterotoxin, Staphylococcus aureus is a highly infectious pathogen and is a considerable threat to food hygiene and safety. Clinical mastitis models were established by S. aureus nipple-tube perfusion. The influence of mastitis on the mammary-gland-tissue proteomic profile was investigated using isobaric tags for relative and absolute quantitation. In this study, healthy and mastitic tissues from different mammary-gland areas of the same dairy cows were screened, and differentially expressed proteins were identified. Bioinformatic analysis identified proteins related to the inflammation and immunization of dairy cows. Histology, immunoblotting, and immunohistochemical-staining analyses were used to determine the expression of PGLYRP1 and PTX3 proteins in the acquired mammary-gland-tissue samples. PGLYRP1 and PTX3 in mastitic mammary glands may be associated with tissue damage and immune responses to late stages of infection. This further contributes to the understanding of the molecular theory of the treatment of mastitis caused by S. aureus.
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