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Disruption of PTPS Gene Causing Pale Body Color and Lethal Phenotype in the Silkworm, Bombyx mori
Xiaoling Tong1, Pingfeng Liang2, Songyuan Wu3
1State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericulture Biology and Genetic Breeding, Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing 400715, China. xltong@swu.edu.cn.
Abstract:
Phenylketonuria (PKU) is an inborn error of metabolism caused by mutations in the phenylalanine hydroxylase (PAH) gene or by defects in the tetrahydrobiopterin (BH4) synthesis pathway. Here, by positional cloning, we report that the 6-pyruvoyl-tetrahydropterin synthase (PTPS) gene, encoding a key enzyme of BH4 biosynthesis, is responsible for the al (albino C) mutation that displays pale body color, head shaking, and eventually lethality after the first molting in silkworm. Compared to wild type, the al mutant produced more substrates (phenylalanine (Phe) and tyrosine (Tyr)) and generated less DOPA and dopamine. Application of 2,4-diamino-6-hydroxypyrimidine (DAHP) to block BH4 synthesis in the wild type effectively produced the al-like phenotype, while BH4 supplementation rescued the defective body color and lethal phenotype in both al and DAHP-treated individuals. The detection of gene expressions and metabolic substances after drugs treatments in al and normal individuals imply that silkworms and humans have a high similarity in the drugs metabolic features and the gene pathway related to BH4 and the dopamine biosynthesis. We propose that the al mutant could be used as an animal model for drug evaluation for BH4-deficient PKU.
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