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A NIR sensor for cyanide detection and its application in cell imaging.

Wei-Na Wu1, Hao Wu1, Yuan Wang1

  • 1College of Chemistry and Chemical Engineering, Henan Key Laboratory of Coal Green Conversion, Henan Polytechnic University, Jiaozuo 454000, PR China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|March 30, 2018
PubMed
Summary

A new sensor detects cyanide (CN-) by observing near-infrared fluorescence quenching. This method, confirmed by advanced techniques, allows for sensitive cyanide determination in biological cells.

Keywords:
Cyanide (CN(−))Fluorescent sensorIntramolecular Charge TransferNear-infrared emissionNucleophilic addition

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Area of Science:

  • Chemical Sensing
  • Organic Synthesis
  • Biophysical Chemistry

Background:

  • Cyanide (CN-) is a highly toxic ion requiring sensitive detection methods.
  • Near-infrared (NIR) fluorescence offers advantages for biological imaging due to lower tissue autofluorescence.
  • Developing selective and sensitive chemosensors for biologically relevant analytes is crucial.

Purpose of the Study:

  • To design and synthesize a novel 'D-π-A' (Donor-π-Acceptor) sensor for cyanide detection.
  • To investigate the sensing mechanism of the sensor towards cyanide.
  • To evaluate the sensor's applicability for cyanide determination in biological samples.

Main Methods:

  • Synthesis of the 'D-π-A' sensor via condensation reaction.
  • Spectroscopic analysis (MS, IR, 1H NMR) for structural confirmation.
  • Density Functional Theory (DFT) calculations to elucidate the sensing mechanism.
  • Fluorescence spectroscopy to monitor the response to cyanide.
  • Application in HeLa cells for cyanide determination.

Main Results:

  • A novel 'D-π-A' sensor was successfully synthesized.
  • The sensor exhibited significant near-infrared (NIR) fluorescence quenching at 663nm upon interaction with cyanide.
  • The response mechanism involves nucleophilic addition of cyanide, inhibiting the Intramolecular Charge Transfer (ICT) process.
  • The sensor was effectively utilized for the determination of cyanide ions in HeLa cells.

Conclusions:

  • The developed 'D-π-A' sensor provides a sensitive and selective method for cyanide detection.
  • The fluorescence quenching mechanism is well-understood through experimental and computational studies.
  • The sensor demonstrates potential for real-world applications in biological imaging and diagnostics.