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Related Concept Videos

Mutations01:39

Mutations

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Overview
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Mutations01:35

Mutations

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Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
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Mutation, Gene Flow, and Genetic Drift01:09

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In a population that is not at Hardy-Weinberg equilibrium, the frequency of alleles changes over time. Therefore, any deviations from the five conditions of Hardy-Weinberg equilibrium can alter the genetic variation of a given population. Conditions that change the genetic variability of a population include mutations, natural selection, non-random mating, gene flow, and genetic drift (small population size).
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Viral Mutations00:36

Viral Mutations

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A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
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Point and Frameshift Mutations01:30

Point and Frameshift Mutations

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Point mutations are genetic alterations involving the change of a single nucleotide base pair in DNA. Depending on how the alteration affects protein synthesis, they can lead to various consequences.Point mutations fall into the following types:Silent mutations occur when a nucleotide change does not alter the amino acid sequence due to the redundancy of the genetic code. For instance, changing ACC to ACA still encodes threonine, leaving the protein function unaffected. This occurs because...
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Mutations in Microorganisms01:18

Mutations in Microorganisms

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Mutations are heritable changes in an organism’s genome involving alterations in the base sequence of DNA or RNA. These changes can influence cellular processes and phenotypic traits, potentially transforming the unaltered wild type into a mutant form. Such changes, termed forward mutations, are pivotal in shaping the genetic diversity of organisms.RNA viruses exhibit the highest mutation rates due to the absence of robust proofreading mechanisms during genome replication. In contrast,...
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Related Experiment Video

Updated: Feb 12, 2026

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation
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Detection of EGFR Gene Mutation by Mutation-oriented LAMP Method.

Naoyuki Matsumoto1, Akira Kumasaka2, Tomohiro Ando2

  • 1Department of Pathology, Tsurumi University School of Dental Medicine, Yokohama, Japan matsumoto-n@tsurumi-u.ac.jp.

Anticancer Research
|March 31, 2018
PubMed
Summary
This summary is machine-generated.

A new loop-mediated isothermal amplification (LAMP) method rapidly detects epidermal growth factor receptor (EGFR) gene mutations at codons 746-753. This advancement aids in personalized treatment planning for non-small cell lung cancer patients.

Keywords:
EGFR mutationLAMPmolecular targeted therapystratification biomarker

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Epidermal growth factor receptor (EGFR) is a key therapeutic target in non-small cell lung cancer (NSCLC).
  • Specific EGFR gene mutations (codons 746-753) are linked to poor prognosis but enhance tyrosine kinase inhibitor efficacy.
  • Accurate detection of these mutations is crucial for guiding NSCLC treatment strategies.

Purpose of the Study:

  • To develop a rapid and sensitive method for detecting EGFR gene mutations at codons 746-753.
  • To establish a mutation-oriented assay for improved NSCLC treatment planning.

Main Methods:

  • A loop-mediated isothermal amplification (LAMP) based procedure was developed.
  • Peptide nucleic acid was utilized to specifically suppress wild-type allele amplification.
  • Six cancer cell lines were analyzed for EGFR gene mutations at codons 746-753.

Main Results:

  • The mutation-oriented LAMP assay successfully amplified the target EGFR gene fragment with mutations within 30 minutes.
  • Boiled cells could be used directly as template DNA, simplifying sample preparation.
  • The assay demonstrated sensitivity when used with extracted DNA.

Conclusions:

  • The developed mutation-oriented LAMP assay provides a rapid and sensitive method for detecting specific EGFR gene mutations.
  • This technique has the potential for chairside detection of EGFR mutations in various clinical samples like sputum and biopsies.
  • This assay can significantly aid in personalized treatment decisions for NSCLC patients.