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Reduction of circulating suppressor inducer T lymphocytes in the exacerbation phase of remitting-relapse multiple
A Cafaro1, M Spadaro, F Pandolfi
1Cattedra di Allergologia ed Immunologia Clinica, Università La Sapienza, Roma.
Abstract:
We studied peripheral blood mononuclear cells (PBMC) from 34 multiple sclerosis (MS) patients: 8 had chronic progressive (CP) and 26 with a relapsing-remitting (RR) course. PBMC were tested with a panel of monoclonal antibodies (MoAbs) including anti-CD3, CD4, CD8, CD25 reagents. In addition, the suppressor inducer lymphocyte subset was investigated by using two color staining with anti-CD4 and G1-15 and/or anti-CD4 and anti-Leu-8 MoaAbs. A significant decrease of the suppressor inducer subset was found in the exacerbation phase of the RR form. Furthermore, a significant decrease of CD8+ (suppressor/cytotoxic) cells was shown in the remission phase of the RR group. Finally, CD25+ lymphocytes were significantly increased in both phases of RR form.
Insights
Multiple sclerosis (MS) patients showed decreased suppressor inducer cells during exacerbation and reduced CD8+ cells during remission. Increased CD25+ lymphocytes were observed in both phases of the relapsing-remitting MS form.
Area of Science:
- Immunology
- Neurology
Background:
- Multiple sclerosis (MS) is a chronic inflammatory disease affecting the central nervous system.
- Understanding immune cell dynamics in different MS disease courses is crucial for therapeutic development.
Purpose of the Study:
- To investigate the changes in specific lymphocyte subsets in patients with relapsing-remitting (RR) and chronic progressive (CP) multiple sclerosis.
- To correlate these changes with disease activity, specifically exacerbation and remission phases in RRMS.
Main Methods:
- Peripheral blood mononuclear cells (PBMC) were analyzed from 34 MS patients (8 CP, 26 RR).
- Flow cytometry was used with monoclonal antibodies (MoAbs) against CD3, CD4, CD8, CD25, and Leu-8.
- The suppressor inducer subset (CD4+CD25+CD69+ or CD4+Leu-8+) was specifically evaluated.
Main Results:
- A significant decrease in the suppressor inducer subset was observed during the exacerbation phase of RRMS.
- A significant reduction in CD8+ (suppressor/cytotoxic) cells was noted during the remission phase of RRMS.
- CD25+ lymphocytes showed a significant increase in both exacerbation and remission phases of RRMS.
Conclusions:
- The study identifies distinct alterations in immune cell populations during different phases of relapsing-remitting multiple sclerosis.
- These findings suggest a role for suppressor inducer cells and CD8+ T cells in MS immunopathogenesis and disease course.
- The increase in CD25+ lymphocytes indicates ongoing immune activation in RRMS.