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Updated: Feb 12, 2026
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Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
Published on: May 26, 2016
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Optimizing T Cell Expansion in a Hollow-Fiber Bioreactor.
Brian Nankervis1, Mark Jones1, Boah Vang1
1Terumo BCT, Inc., 11158 West Collins Avenue, Lakewood, CO 80215 USA.
Current Stem Cell Reports
|March 31, 2018
Summary
Hollow-fiber bioreactors enable large-scale expansion of gene-modified human T cells. This method overcomes nutrient and waste limitations, significantly increasing cell yield compared to traditional methods.
Area of Science:
- Regenerative Medicine
- Cell Therapy
- Bioprocessing
Background:
- Traditional cell culture methods (well-plate and flask-based) are insufficient for the large-scale expansion of gene-modified human T cells required by regenerative medicine.
- Cell proliferation is often limited by nutrient and oxygen supply and waste product accumulation in static culture environments.
Purpose of the Study:
- To review process development changes necessary for meeting high-yield T cell expansion demands.
- To discuss the utilization of hollow-fiber bioreactors for overcoming limitations in cell expansion.
Main Methods:
- Utilizing hollow-fiber bioreactors for cell culture.
- Implementing perfusion feeding strategies.
- Enhancing gas exchange and waste removal (lactate).
Main Results:
- Perfusion feeding, improved gas exchange, and lactate removal increased T cell yield from 10.8E+09 to over 28E+09 cells within 10 days.
- Active cell retention in the bioreactor improved environmental management.
Conclusions:
- Hollow-fiber bioreactors, with optimized feeding and environmental control, can overcome constraints limiting cell expansion.
- This technology facilitates significantly higher yields of gene-modified T cells for therapeutic applications.
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