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Updated: Feb 12, 2026

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
Published on: January 7, 2019
Measurements of Intra-oocyte Nitric Oxide Concentration Using Nitric Oxide Selective Electrode
Sana N Khan1, Roohi Jeelani1, Husam M Abu-Soud2,3
1Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Wayne State University, Detroit, MI, USA.
Abstract:
Precise information about the intracell nitric oxide (NO) concentration [NO] of a single cell are necessary in designing accurate experiments to further knowledge and develop treatment plans in certain disorders. The direct quantitative measurement of [NO] in situ in an intact cellular complex should be useful in tracking real-time and rapid changes at nanomolar levels. In this work, we describe the direct, real-time, and quantitative intracellular [NO] measurement utilizing an L-shaped amperometric integrated NO-selective electrode. This method not only provides an elegant and convenient approach to real-time the measurement of NO in physiological environments but also mimics the loss of NO caused by rapid NO diffusion combined with its reactivity in the biological milieu.
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