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Surface Plasmon Resonance Spectroscopy for Detection of S-Nitrosylated Proteins
Lili Zhang1, Pengpeng Shang1, Changbao Chen1
1College of Chemistry and Material Science, Shandong Agricultural University, Taian, China.
Abstract:
Protein S-nitrosylation, the NO-dependent regulatory mechanism, is a posttranslational modification of reactive cysteine thiols to form S-nitrosothiols. The biotin-switch technique (BST) has become a mainstay method for detection of S-nitrosylated proteins in biological samples. On the basis of BST, we describe a surface plasmon resonance (SPR) spectroscopy method for detecting S-nitrosylated proteins. This method can be applied for the indirect determination of S-nitrosylated proteins in biological samples.
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