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Morphological features of doxorubicin-resistant Walker 256 carcinosarcoma and response of mast cells

N V Boroday1, V F Chekhun1

  • 1R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.

Experimental Oncology
|March 31, 2018
PubMed
Abstract

Insights

Mast cells (MCs) play a role in doxorubicin (DOX) resistance development in Walker 256 carcinosarcoma. Their numbers and histamine content initially increase with DOX resistance but decrease in fully resistant tumors.

Area of Science:

  • Oncology
  • Immunology
  • Pharmacology

Background:

  • Mechanisms of cancer drug resistance remain incompletely understood.
  • Mast cells (MCs) are increasingly recognized for their role in drug resistance.
  • Doxorubicin (DOX) resistance in cancer is a significant clinical challenge.

Purpose of the Study:

  • To investigate the morphological characteristics of doxorubicin (DOX)-resistant Walker 256 carcinosarcoma.
  • To evaluate the response of mast cells (MCs) and their histamine content in relation to DOX resistance.
  • To analyze MCs and histamine levels in both developing and established DOX-resistant tumors.

Main Methods:

  • DOX resistance was induced in Walker 256 carcinosarcoma via serial in vivo passages with DOX treatment.
  • Mast cells (MCs) in tumor sections were identified using Toluidine Blue O staining.
  • Histamine content in MCs was quantified using the Astaldi semiquantitative method with Water Blue-Orcein staining.

Main Results:

  • Increased numbers of mast cells (MCs) and elevated histamine content were observed during the development of DOX resistance.
  • In tumors exhibiting complete DOX resistance, the count of histamine-containing MCs returned to levels similar to DOX-sensitive tumors.
  • These findings suggest a dynamic role for MCs in the progression of DOX resistance.

Conclusions:

  • Mast cells (MCs) are implicated in the development of doxorubicin (DOX) resistance in Walker 256 carcinosarcoma.
  • The observed changes in MCs and histamine content highlight their potential as modulators of drug resistance.
  • Further research is warranted to elucidate the precise mechanisms by which MCs influence DOX resistance.

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