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Polysome Purification from Soybean Symbiotic Nodules
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Polysome Purification from Soybean Symbiotic Nodules

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Isolation, purification and characterization of exopolysaccharide produced by Leuconostoc pseudomesenteroides YF32

Yanfang Yang1, Fang Feng1, Qingqing Zhou1

  • 1School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.

International Journal of Biological Macromolecules
|March 31, 2018
PubMed

Insights

A novel exopolysaccharide (EPS) from Leuconostoc pseudomesenteroides YF32, identified as a high-molecular-weight dextran, demonstrates excellent thermal stability and solubility. This EPS shows potential as a functional food additive.

Area of Science:

  • Food Science
  • Microbiology
  • Biochemistry

Background:

  • Exopolysaccharides (EPS) are microbial polymers with diverse applications.
  • Soybean paste is a potential source of novel EPS-producing microorganisms.
  • Leuconostoc species are known for their EPS production capabilities.

Purpose of the Study:

  • To isolate and identify an EPS-producing strain from soybean paste.
  • To characterize the structure, properties, and potential applications of the produced EPS.
  • To evaluate the suitability of the EPS as a food additive.

Main Methods:

  • Isolation and identification of the EPS-producing strain YF32 (Leuconostoc pseudomesenteroides).
  • Cultivation in Man-Rogosa-Sharpe (MRS) medium with sucrose.
  • Purification and characterization of EPS using High-Performance Size-Exclusion Chromatography (HPSEC), Gas Chromatography (GC), Fourier Transform Infrared (FT-IR), and Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Determination of thermal stability and functional properties (solubility, emulsibility).

Main Results:

  • A yield of 12.5 g/L EPS was achieved.
  • The EPS was identified as a dextran with a weight-average molecular weight (Mw) of 5.54×10^6 Da.
  • Structural analysis revealed a linear backbone of α-(1→6)-linked d-glucopyranose units.
  • High thermal stability (degradation temperature of 307.62°C), water solubility, and emulsibility were observed.

Conclusions:

  • The EPS produced by Leuconostoc pseudomesenteroides YF32 is a stable, water-soluble dextran.
  • The characterized dextran exhibits properties suitable for food applications.
  • This dextran has significant potential for use as a food additive.
Keywords:
CharacterizationDextranExopolysaccharideLeuconostoc pseudomesenteroidesStructure

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