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Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
Published on: March 29, 2018
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Bone morphogenetic protein-2 release profile modulates bone formation in phosphorylated hydrogel.
Maurits G L Olthof1,2,3,4, Diederik H R Kempen5, Xifeng Liu1,2
1Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, MN, USA.
Journal of Tissue Engineering and Regenerative Medicine
|April 1, 2018
Summary
Bone morphogenetic protein-2 (BMP-2) burst release from novel hydrogel composites significantly enhances bone formation compared to sustained release, outperforming current clinical standards.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- The optimal release kinetics of bone morphogenetic protein-2 (BMP-2) for bone regeneration remain unclear.
- Novel biomaterials are needed to control BMP-2 delivery for enhanced clinical efficacy.
Purpose of the Study:
- To investigate the impact of differential BMP-2 release profiles on bone formation using a novel hydrogel composite.
- To compare the efficacy of different BMP-2 loading strategies with a clinical standard.
Main Methods:
- Three composite implants (OPF-BP-Msp, OPF-BP-Cmb, OPF-BP-Ads) were fabricated using oligo[(polyethylene glycol) fumarate] bis[2-(methacryloyloxy) ethyl] phosphate (OPF-BP) hydrogel and poly(lactic-co-glycolic acid) microspheres.
- Differential BMP-2 release profiles were characterized.
- Bone formation was assessed in a rat subcutaneous implantation model and compared to absorbable collagen sponge (ACS).
Main Results:
- OPF-BP-Ads and ACS exhibited rapid BMP-2 burst release (>75%), while OPF-BP-Msp and OPF-BP-Cmb showed sustained release (~25% and ~50% burst, respectively).
- OPF-BP-Ads induced significantly greater bone formation, exceeding that of ACS by 12-fold.
- Burst release of BMP-2 from OPF-BP composites resulted in more bone formation than sustained release.
Conclusions:
- BMP-2 burst release is more effective for inducing subcutaneous bone formation than sustained release within the studied OPF-BP composite system.
- Biomaterial composites must provide both controlled drug delivery and a suitable structural framework for optimal bone regeneration.
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