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Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Published on: June 26, 2010
A benzothiazole-based fluorescent probe for hypochlorous acid detection and imaging in living cells
Khac Hong Nguyen1, Yuanqiang Hao2, Ke Zeng3
1College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China; Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, Hunan 476000, PR China.
Abstract:
A benzothiazole-based turn-on fluorescent probe with a large Stokes shift (190nm) has been developed for hypochlorous acid detection. The probe displays prompt fluorescence response for HClO with excellent selectivity over other reactive oxygen species as well as a low detection limit of 0.08μM. The sensing mechanism involves the HClO-induced specific oxidation of oxime moiety of the probe to nitrile oxide, which was confirmed by HPLC-MS technique. Furthermore, imaging studies demonstrated that the probe is cell permeable and can be applied to detect HClO in living cells.
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