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Updated: Feb 12, 2026

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy
Published on: September 28, 2022
Note: Eliminating stripe artifacts in light-sheet fluorescence imaging
S M Salili1, M Harrington1, D J Durian1
1Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Abstract:
We report two techniques to mitigate stripe artifacts in light-sheet fluorescence imaging. The first uses an image processing algorithm called the multidirectional stripe remover method to filter stripes from an existing image. The second uses an elliptical holographic diffuser with strong scattering anisotropy to prevent stripe formation during image acquisition. These techniques facilitate accurate interpretation of image data, especially in denser samples. They are also facile and cost-effective.
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