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Related Concept Videos

RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Interference and Diffraction02:18

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Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
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Interference and Decay01:16

Interference and Decay

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Forgetting is a complex cognitive phenomenon influenced by several factors, among which interference and decay are particularly prominent. These processes explain why individuals often struggle to retrieve specific information from memory, leading to lapses in recall that can be observed in everyday situations.
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RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

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Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
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RNA Interference in Ticks
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CRISPR/Cas13 as a Tool for RNA Interference.

Zahir Ali1, Ahmed Mahas1, Magdy Mahfouz1

  • 1Laboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.

Trends in Plant Science
|April 2, 2018
PubMed
Summary
This summary is machine-generated.

CRISPR/Cas13 technology enables precise RNA manipulation in plants, offering new ways to study and control biological processes. This review explores the potential and challenges of using this powerful tool for transcriptome engineering in plant science.

Keywords:
CRISPR/Cas13RNA manipulationtranscriptome regulationviral interference

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Area of Science:

  • Molecular Biology
  • Plant Science
  • Biotechnology

Background:

  • RNA plays a fundamental role in nearly all biological processes.
  • Efficient tools for transcriptome manipulation are essential for biological research.
  • The bacterial CRISPR/Cas13 system has been adapted for RNA targeting.

Purpose of the Study:

  • To review the applications of the CRISPR/Cas13 system for RNA manipulation in plants.
  • To discuss the opportunities and limitations of CRISPR/Cas13 in plant transcriptome engineering.
  • To highlight the potential impact of CRISPR/Cas13 on plant biology research.

Main Methods:

  • Review of existing literature on CRISPR/Cas13 systems in plants.
  • Analysis of reported methods for RNA manipulation using CRISPR/Cas13.
  • Discussion of the advantages and disadvantages of CRISPR/Cas13 for plant applications.

Main Results:

  • CRISPR/Cas13 systems can be effectively rewired for RNA manipulation in eukaryotic systems, including plants.
  • Various RNA manipulation strategies can be achieved using CRISPR/Cas13, such as knockdown and editing.
  • The system presents significant opportunities for advancing plant science research and biotechnology.

Conclusions:

  • CRISPR/Cas13 offers a versatile platform for targeted RNA manipulation in plants.
  • Understanding the limitations is crucial for successful implementation in plant research.
  • This technology holds great promise for future advancements in plant biology and crop improvement.