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Related Experiment Video

Updated: Feb 12, 2026

Candida albicans Biofilm Chip CaBChip for High-throughput Antifungal Drug Screening
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Technical note: High-throughput method for antifungal activity screening in a cheese-mimicking model.

Lucille Garnier1, Marcia Leyva Salas1, Nicolas Pinon2

  • 1Université de Brest, EA 3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, ESIAB, Technopôle Brest-Iroise, 29280 Plouzané, France; STLO, Agrocampus Ouest, INRA, 35000 Rennes, France.

Journal of Dairy Science
|April 2, 2018
PubMed
Summary

A new high-throughput method screens antifungal agents using a cheese-mimicking matrix. This rapid technique evaluates lactic acid bacteria (LAB) and preservatives against fungi, revealing strain-dependent activity.

Keywords:
antifungal activitycheese modelhigh-throughput screening

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Area of Science:

  • Food microbiology
  • Antimicrobial screening
  • Dairy technology

Background:

  • Antifungal agents are crucial for food preservation.
  • Existing screening methods can be slow and resource-intensive.
  • Lactic acid bacteria (LAB) show potential as natural antifungal agents.

Purpose of the Study:

  • To develop a high-throughput method for screening antifungal activity.
  • To evaluate the antifungal potential of LAB and preservatives in a food-relevant matrix.
  • To assess the influence of fermentation substrate and screening medium on antifungal efficacy.

Main Methods:

  • A cheese-mimicking matrix in 24-well plates was used for high-throughput screening.
  • 44 LAB fermented milk-based ingredients and 23 LAB isolates were tested against four fungal species.
  • Minimum inhibitory concentrations (MICs) of natamycin were determined against nine fungal targets.

Main Results:

  • The method achieved a screening rate of 1,600 assays per week.
  • LAB antifungal activity was strain-dependent and influenced by the fermentation substrate.
  • The screening medium affected natamycin's MIC, highlighting matrix effects.

Conclusions:

  • The developed method enables rapid, high-throughput screening of antifungal agents in a dairy-compatible matrix.
  • It effectively characterizes the antifungal activity of LAB and preservatives.
  • This approach is valuable for identifying novel antimicrobial compounds for food applications.