Analysis of the Ca2+-binding parvalbumin in rat skeletal muscles of different thyroid states

The Ca2+-binding parvalbumin (PV) is possibly involved in the relaxation of fast-twitch muscle fibers and believed to be a marker for early muscular disturbances. The muscular content of parvalbumin has been shown to change with alterations of the relaxation speed that follow an experimentally changed nervous input. In hypo- and hyperthyroidism isometric twitch contraction and half-relaxation times are also altered, namely increased in hypothyroidism and decreased in hyperthyroidism. These changes are largely paralleled by modifications in the fiber type composition. Therefore we investigated the distribution and concentration of parvalbumin in extensor digitorum longus, soleus, and gastrocnemius muscles of rats by immunohistochemical and biochemical methods. The combined results of both procedures showed that parvalbumin distribution and concentration were largely unaffected in all thyroid states. This suggests that the expression of parvalbumin is neuronally controlled and not by thyroid hormones. Additionally our findings support the view that the changes in physiologic properties and fiber type composition are generated by a direct action of thyroid hormone on muscle fibers, and not via their nervous input.